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Etude multi-échelle de l'interaction cellule-biomatériau par microscopie confocale et environnementale

Abstract : The success of the development of new implants relies on a better understanding of the interactions between cells and biomaterials. Cell adhesion on the biomaterial is a key step in predicting the future of an implant in the human body. Cell adhesion is involved in many cellular processes such as proliferation, migration and differentiation. Recent studies have shown that the substrate on which cells are grown can lead to different cell morphologies. This thesis work consisted in setting up a method for studying cell adhesion by quantifying the anchoring sites of the cell on the biomaterial by confocal microscopy. The morphological study was carried out by scanning electron microscopy (SEM). Morphological analysis is mainly performed in 2D using high vacuum SEM (HVSEM), which notably requires cell dehydration. We initially tried to develop a 3D analysis method of the cell on the biomaterial by SEM. Then, we developed a 3D analysis method to study whole cells close to their native state. To do so, wet-STEM was performed using a tomographic sample holder designed beforehand in the laboratory to study aqueous suspensions in 3D. This specific rack allows analyzes by collecting the electrons transmitted or diffused through the sample. Such development opens the way to 3D analysis of intact cells in environments mimicking their native environment.
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https://theses.hal.science/tel-03783227
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Submitted on : Thursday, September 22, 2022 - 9:16:27 AM
Last modification on : Friday, September 30, 2022 - 11:40:09 AM

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  • HAL Id : tel-03783227, version 1

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Akkiz Bekel. Etude multi-échelle de l'interaction cellule-biomatériau par microscopie confocale et environnementale. Matériaux. Université de Lyon, 2022. Français. ⟨NNT : 2022LYSEI033⟩. ⟨tel-03783227⟩

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