Low vision affects the ability to read and can be a major barrier to educational success and insertion into the workplace. We designed a new font (Luciole) to improve the readability and comfort for people with low vision. In this study, we analyze the effect of the font type on readability. Luciole was compared to five other fonts (Arial, OpenDyslexic, Verdana, Eido and Frutiger) in 145 French readers (73 with low vision, and 72 normal sighted), aged 6 to 35 years old and divided into four reading expertise groups. Participants completed two tasks, first reading texts on paper and then reading false-words on screen using eye tracking. About half of the participants with low vision had a subjective preference for Luciole when reading on paper and on the screen; lower preference is noted for participants with normal vision. Other readability criteria show a slight advantage of the Luciole font over some fonts (e.g., Eido and OpenDyslexic) in both groups. The results obtained when taking into consideration the level of reading expertise confirm this trend.