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Purification of dirucotide, a synthetic 17-aminoacid peptide, by ion exchange centrifugal partition chromatography

Abstract : Dirucotide is a synthetic drug candidate for the treatment of multiple sclerosis. This 17-aminoacid peptide was successfully purified by ion exchange centrifugal partition chromatography. The optimized conditions involved the biphasic methyl tert-butyl ether/acetonitrile/n-butanol/water (2:1:2:5, v/v) solvent system in the descending mode, the di(2-ethylhexyl)phosphoric acid cation-exchanger with an exchanger (di(2-ethylhexyl)phosphoric acid)/dirucotide mole ratio of 100 and Ca2+ ions in aqueous solution as displacer. Critical impurities were efficiently eliminated and dirucotide was recovered in high yield and purity (69% and 98%, respectively) and with a productivity of 2.29 g per liter of stationary phase per hour.
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Submitted on : Wednesday, September 22, 2021 - 11:38:11 AM
Last modification on : Thursday, October 14, 2021 - 1:10:09 PM

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Leslie Boudesocque, Luciano Forni, Agathe Martinez, Jean-Marc Nuzillard, Matthieu Giraud, et al.. Purification of dirucotide, a synthetic 17-aminoacid peptide, by ion exchange centrifugal partition chromatography. Journal of Chromatography A, Elsevier, 2017, 1513, pp.78-83. ⟨10.1016/j.chroma.2017.07.003⟩. ⟨hal-01608841⟩

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